He adopted the European model of museums, bringing it to the United States through his founding roles in the Metropolitan Museum of Art and the Everson Museum of Art (formerly the Syracuse Museum of Fine Arts). Comfort's artistic signature is evident in his paintings, which demonstrate a keen eye for detail and a deep understanding of composition.

His notable works include those that showcase American landscapes and cityscapes, reflecting his fascination with the country's diverse geography and culture. As one of the founders of two prominent museums, Comfort played a pivotal role in shaping America's artistic landscape. His legacy extends beyond his own artwork to the countless artists and art enthusiasts he inspired through his contributions to museum development.

Through his dedication to showcasing American art, Comfort left an enduring impact on the country's cultural heritage.